Antioxidant Effect of Hibiscus sabdariffa Calyces Aqueous Extract Against Sleep Deprivation-induced Pituitary, Testicular and Epidydimal Oxidative Damage in Wistar Rats

Authors

  • Ibrahim Musah Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Nachamada Emmanuel Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Rukayya Abdulrauf Adebisi Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Hajara Ali Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Baraka Umar Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Fati Dautiya Malgwi Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Siraj Nuruddeen Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Oluwakemi Mary Olowomeye 2Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Achievers University, Owo, Ondo State. Nigeria
  • Anami Emmanuel Adamawa State College of Nursing and Midwifery, P.M.B 2044 Yola. Adamawa State, Nigeria
  • Favour Obuetor Wisdom Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Amina Shehu Auwalu Department of Human Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria
  • Olufunke Onaadepo Department of Physiology, Faculty of Basic Medical Sciences, University of Abuja, Abuja. NIgeria

DOI:

https://doi.org/10.33003/sajols-2025-0302-19

Abstract

Sleep deprivation can significantly impact male fertility. Ensuring sufficient and quality sleep is crucial for maintaining optimal reproductive health. This study evaluated the effect of Hibiscus sabdariffa calyces against sleep deprivation-induced testicular and epididymal toxicity in Wistar rats. Thirty male Wistar rats were divided into six groups of seven animals each, with group I as the control receiving distilled water, group II as sleep-deprived and untreated, and Groups III-V receiving varying doses of HS (100, 200, and 400 mg/kg), respectively, while being sleep-deprived. Group VI received vitamin C (100 mg/kg) and was sleep-deprived. Administration of HS significantly (P< 0.05) reduced testicular and epididymal malondialdehyde (MDA) compared to the sleep-deprived-untreated group. Antioxidant enzymes; SOD, GSH CAT and GSH were significantly increased (P< 0.05) with HS treatment compared to the sleep-deprived-untreated group. These results support the potential of Hibiscus sabdariffa as a natural therapeutic agent in mitigating sleep deprivation-induced reproductive toxicity.

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Published

2025-06-30

How to Cite

Musah, I., Emmanuel, N., Adebisi, R. A., Ali, H., Umar, B., Malgwi, F. D., Nuruddeen, S., Olowomeye, O. M., Emmanuel, A., Wisdom, F. O., Auwalu, A. S., & Onaadepo, O. (2025). Antioxidant Effect of Hibiscus sabdariffa Calyces Aqueous Extract Against Sleep Deprivation-induced Pituitary, Testicular and Epidydimal Oxidative Damage in Wistar Rats. Sahel Journal of Life Sciences FUDMA, 3(2), 154–164. https://doi.org/10.33003/sajols-2025-0302-19

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