Antibacterial and Antibiofilm efficacy of Lactobacillus plantarum cell-free extract against some multidrug resistant bacterial pathogens associated with chronic wounds

Abstract

• Abstract

  The existence of multidrug-resistant bacterial pathogens and biofilm formation are critical factors that compromise the management of wound infections and pose significant barriers to successful therapeutic outcomes. The study aimed to evaluate the antibacterial and antibiofilm effect of L. plantarum cell-free supernatant (CSF) against resistant strains of S. aureus and P. aeruginosa biofilms associated with chronic wounds. Antibacterial susceptibility testing was performed to determine the resistance profile of S. aureus and P. aeruginosa. Antibacterial activity was evaluated using the agar well diffusion method at various concentrations of CSF. Crystal violet microtiter plate assay was used to quantify the antibiofilm activity of the isolates. A high level of resistance to penicillin and ofloxacin was observed among the isolates. Significant antibacterial effects were observed, with inhibition zones of 17.2 mm for S. aureus at 100 µL (p = 0.002) and 19.7 mm for P. aeruginosa at 200 µL (p = 0.018). Quantification of biofilm revealed OD₆₀₀ values of 0.48 and 0.72, corresponding to 60% and 46.7% biofilm inhibition for S. aureus and P. aeruginosa, respectively. The findings highlight promising antibacterial and antibiofilm activity of L. plantarum CSF bioactive against multidrug-resistant pathogens associated with chronic wounds, suggesting its potential as a potential therapeutic agent.