Cells Adhesion Inhibitory Activity of Identified Compounds from Taminalia catappa Against Clinical Isolate of Trichosporon asahi

Abstract

Trichosporon infection represents an emerging and still underestimated type of fungal infection that can lead to severe medical manifestations. It is associated with a high mortality rate worldwide, especially among immunocompromised patients. Trichosporon asahi rapidly forms biofilm which are cells composed of extracellular matrix. This biofilm formation by cell adherence to surfaces is a mechanism of antibiotic resistance employed by T. asahi. This research work is aimed at determining the adhesion-inhibitory activity of identified compounds from T. catappa against a clinical isolate of Trichosporon asahi. The microdilution method was used to form adhered cells on the microtitre wells and Column chromatography was used to fractionate the n-butanol extract of T. catappa. The wells containing nitrogen-based broth were inoculated with a cell suspension of Trichosporon asahi isolate and treated with fractions of N-butanol extracts of T. catappa. Fraction (F8) significantly inhibited the formation of adhered cells compared to other fractions, which is reflected in the concentration of the adhered cells formed. GC-MS of the F8 revealed various compounds with methyl ester having 99% purity.  F8 fraction of T. catappa contains compounds most especially methyl ester, with 99% purity that disrupts the formation of adhered cells, therefore reducing antibiotics resistance.